Strange the way 'you' and 'thou' have effectively changed placed in contemporary English usage. 'You' is now familiar, where 'thou' sounds archaic and stiffly formal and therefore more mannered and respectful. Of course, strictly speaking, 'you' is polite/formal and 'thou' de haut en bas, cheeky, friendly or intimate.
The prize of an orange to whomsover identifies the source of the title quotation. Suffice to say, it's from a popular culture text, and is uttered by a respectful junior to a powerful senior in a particular heirarchy.